Figure 5: Multiple structurally distinct putative T-type (but not L-type) calcium channel blockers suppress Angiopoietin-2 (Angpt-2).
From: Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis
10 μM Flunarizine (FLU) or vehicle was applied for 24 hrs after pretreatment with (A) 10 μM Mibefradil or control for 1 h, (B) 50 μM TTA-A2 (a specific pharmacological inhibitor of t-type CCs) or control for 1 h, (C) 10 μM Amlodipine or control for 1 h. Angpt-2 concentration in the supernatant of human umbilical vein endothelial cells (HUVECs) was measured by enzyme-linked immunosorbent assay (ELISA) (n = 6–10). (D) Before application of 10 μM FLU or vehicle for 24 hrs HUVECs were pretreated with 1 μM Thapsigargin or control for 0.5 hrs. Angpt-2 concentration in the supernatant was measured by ELISA and is shown relative to whole intracellular protein (n = 6–10). (E) 10 μM FLU or control was applied for 8 hrs after pretreatment with 10 μM EDTA for 1 h and the concentration of Angpt-2 in the supernatant was determined by ELISA (n = 6). Columns are presented as mean ± SEM.
