Figure 1: In situ hybridization and real-time PCR analysis of miR-153. | Scientific Reports

Figure 1: In situ hybridization and real-time PCR analysis of miR-153.

From: MiR-153 Regulates Amelogenesis by Targeting Endocytotic and Endosomal/lysosomal Pathways–Novel Insight into the Origins of Enamel Pathologies

Figure 1

Am: Ameloblast; En: Enamel; De: Dentin; Od: Odontoblast; ES: Enamel Space. M-S: Molar-Secretory stage; M-M: Molar-Maturation stage; I-P: Incisor-Presecretory stage; I-S: Incisor-Secretory stage; I-M: Incisor-Maturation stage. **Marks the signals from positive control (a–e) and miR-153 expression (a′–e′). (a,a′) secretory-stage enamel organ (PN6) of mouse mandibular first molar; (b,b′) maturation-stage enamel organ (PN9) of mouse mandibular first molar; (c,c′) presecretory-stage enamel organ of PN9 mouse mandibular incisor; (d,d′) secretory-stage enamel organ of PN9 mouse mandibular incisor; (e,e′) maturation-stage enamel organ of PN9 mouse mandibular incisor. Positive control for in situ hybridization is shown in panels (a–e) where the samples were incubated with U6 detection probes, and signals were mainly observed in the nuclei. MiR-153 expression by in situ hybridization is shown in panels a′–e′. The miR-153 signals developed from presecretory- and secretory-stage enamel organ (a′,c′,d′) were higher than those from maturation-stage enamel organ (b′,e′). The scale bar for all in situ hybridization images was 100 μm. Relative expression of miR-153 by real-time PCR using total RNAs isolated from secretory- and maturation-stage mandibular first molars of four animals (n = 4; panel f). The expression of miR-153 was significantly downregulated at maturation stage compared with secretory stage (*P = 0.002). The average fold change of miR-153 was ~−3.8 (maturation/secretory; panel g).

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