Figure 4: MiR-153 induced changes in gene expression at protein level by western blot. | Scientific Reports

Figure 4: MiR-153 induced changes in gene expression at protein level by western blot.

From: MiR-153 Regulates Amelogenesis by Targeting Endocytotic and Endosomal/lysosomal Pathways–Novel Insight into the Origins of Enamel Pathologies

Figure 4

(a1d1) Protein-level gene expression changes in ALCs. (a2d2) Protein-level gene expression changes in LS8 cells. At protein level, intracellular overloading of miR-153 caused statistically significant downregulation of Clcn4 (a1,a2), Lamp1 (b1,b2), Cltc (c1,c2) and Slc4a4 (d1,d2), which corresponded well with the results from real-time PCR analysis at mRNA level. beta-actin served as controls for sample loading, and band densities were quantified relative beta-actin. *P < 0.05.

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