Figure 5: Luciferase reporter assay verifying the predicted interaction between miR-153 and target genes. | Scientific Reports

Figure 5: Luciferase reporter assay verifying the predicted interaction between miR-153 and target genes.

From: MiR-153 Regulates Amelogenesis by Targeting Endocytotic and Endosomal/lysosomal Pathways–Novel Insight into the Origins of Enamel Pathologies

Figure 5

(a) Luciferase vector construct. (b) Interactions between miR-153 and target genes predicted by TargetScan. Luciferase activities were presented in ratios of Renilla to Firefly luciferase reporter activities. In the luciferase reporter assays of Cltc (c) and Clcn4 (d), miR-153 mimics caused decreases of ~77% and ~46% in the luciferase reporter activities. The differences in luciferase reporter activities from Slc4a4 vectors (e,f) between miR-153 transfection group and mock transfection group were also statistically significant (a fragment: ~60% decrease in luciferase activity; b fragment: ~46% decrease in luciferase activity). *P < 0.05.

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