Table 3 Summary measures for the uptake of 125I-labelled LDL by HepG2 cells in the presence of ex vivo VLDL1 and VLDL2-rich TRL fractions isolated from fasting and postprandial plasma samples.

From: A randomized trial and novel SPR technique identifies altered lipoprotein-LDL receptor binding as a mechanism underlying elevated LDL-cholesterol in APOE4s

 

Fasting

Postprandial

P for ANOVA

APOE3/E3

APOE3/E4

P

APOE3/E3

APOE3/E4

Meal

Genotype

Meal × genotype

SFA

SFA-FO

UNSAT

SFA

SFA-FO

UNSAT

VLDL1-rich

Heparin1

108.2 ± 3.5

49.8 ± 12.1

0.001

63.7 ± 10.0

65.5 ± 9.2

66.1 ± 11.4

51.2 ± 7.3a

82.6 ± 13.4b

84.8 ± 18.5b

0.023

0.63

0.046

Cell associated1

54.9 ± 7.4

59.3 ± 5.9

0.67

77.6 ± 7.8

84.2 ± 8.1

71.8 ± 6.3

66.0 ± 17.8

81.7 ± 17.6

70.4 ± 8.7

0.036

0.74

0.48

Deg. Products1

88.2 ± 8.2

81.2 ± 3.9

0.51

59.9 ± 13.4

66.8 ± 13.3

73.8 ± 15.9

77.8 ± 21.8

89.6 ± 19.3

94.0 ± 20.1

0.17

0.49

0.43

Total uptake1

65.3 ± 5.6

68.5 ± 2.4

0.89

70.1 ± 8.7

76 ± 8.8

69.1 ± 9.8

67.6 ± 8.8a

81.9 ± 8.4b

79.9 ± 9.8b

0.053

0.89

0.07

VLDL2-rich

Heparin

109.2 ± 9.9

75.1 ± 8.3

0.038

61.8 ± 12.8

58.1 ± 10.6

55.0 ± 11.0

62.1 ± 10.3

91.1 ± 34.7

86.9 ± 21.0

0.39

0.37

0.16

Cell assoc

77.0 ± 6.8

88.6 ± 5.9

0.25

54.8 ± 11.7

57.0 ± 13.7

49.9 ± 9.0

56.9 ± 13.8a

89.0 ± 21.9b

70.5 ± 16.9a

0.019

0.38

0.047

Deg products

92.3 ± 7.7

70.9 ± 7.5

0.32

54.4 ± 8.9

44.8 ± 6.3

50.4 ± 6.8

60.5 ± 16.9

94.6 ± 44.7

82.1 ± 29.3

0.12

0.33

0.07

Total uptake

81.9 ± 6.7

81.8 ± 6.3

0.32

54.9 ± 9.5

51.9 ± 10.5

50.6 ± 8.0

55.7 ± 8.2

88.1 ± 26

73 ± 18.2

0.13

0.32

0.07

  1. Data are mean ± SEM for the percentage change in heparin releasable binding, cell associated radioactivity, degradation products and total uptake of 125I-labelled LDL after 5 h in the presence of VLDL1 and VLDL2-rich fractions, compared with 125I-labelled LDL in the absence of TRL. Each experiment was performed in duplicate and represents n = 5 independent experiments for APOE3/E3 and n = 4 independent experiments for APOE3/E4 group.
  2. Heparin- refers to the heparin releasable, cell surface bound 125I-LDL, Cell associated- refers to the 125I-LDL which has been internalized into the cell, Deg. products- refers to the degradation products which have been released by the cell back into the medium, Total uptake is the sum of the cell surface bound (heparin), cell associated and degradation products.
  3. The absolute values for the total uptake of 125I-labelled LDL in the absence of TRL was 948 ± 137 ng/mg cell protein (heparin releasable binding 40 ± 5 ng/mg cell protein, cell associated radioactivity 587 ± 81 ng/cell protein and degradation products 323 ± 37 ng/cell protein) and 881 ± 127 ng/cell protein (heparin releasable binding 38 ± 5 ng/cell protein, cell associated radioactivity 480 ± 76 ng/cell protein and degradation products 363 ± 53 ng/cell protein) for APOE3/E3 and APOE3/E4 LDL, respectively.
  4. In the fasting state, differences between genotype groups were compared using the Student’s independent t-test (P ≤ 0.05). For the TRLs isolated after the saturated fatty acid meal (SFA), SFA meal with fish oil (SFA-FO) and unsaturated fatty acid meal (UNSAT), data were analyzed using a mixed factor ANOVA. When the meal*genotype interaction were significant, one way repeated measures ANOVA followed by Student’s t test with Bonferroni correction (P < 0.017) were used to determine differences between meals within each genotype group. Values with different superscript letters within each row are significantly different.
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