Figure 2: Prolonged activation of NMDA receptors causes β-cell dysfunction in vitro. | Scientific Reports

Figure 2: Prolonged activation of NMDA receptors causes β-cell dysfunction in vitro.

From: An excessive increase in glutamate contributes to glucose-toxicity in β-cells via activation of pancreatic NMDA receptors in rodent diabetes

Figure 2

(a) Effect of different concentrations of NMDA treatment for 72 h on the cell viability of RINm5f β-cells (n = 9, **p < 0.01 vs 0 mM NMDA group). (b) Effect of different concentrations of NMDA treatment for 72 h on the LDH activity in the supernatant of cultured RINm5f β-cells (n = 6, *p < 0.05 vs 0 mM NMDA group; **p < 0.01 vs 0 mM NMDA group). (c) Effect of different concentrations of NMDA treatment for 72 h on the intracellular ATP content of RINm5f β-cells (n = 5, *p < 0.05 vs 0 mM NMDA group; **p < 0.01 vs 0 mM NMDA group). (d) Effect of different concentrations of NMDA treatment for 72 h on insulin secretion from RINm5f β-cells (n = 6, *p < 0.05 vs 0 mM NMDA group). (e) Representative western blot showing the knockout efficiency of NMDAR1 protein in RINm5f cells treated with GluN1 sgRNA (n = 4, *p < 0.05 vs control sgRNA; **p < 0.01 vs control sgRNA group). (f) Insulin secretion from RINm5f cells transfected with sgRNA under stimulation with NMDA (10 mM) for 72 h (n = 6, *p < 0.05 vs control sgRNA, #p < 0.05 vs control sgRNA + NMDA group).

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