Figure 1: Gene amplification, cloning, expression and purification. | Scientific Reports

Figure 1: Gene amplification, cloning, expression and purification.

From: Evaluation of 5 Novel protein biomarkers for the rapid diagnosis of pulmonary and extra-pulmonary tuberculosis: preliminary results

Figure 1

(A) Agarose gel electrophoresis for PCR products. Lane M, 1 kb DNA Molecular-size marker; lane 1, 2: amplification of respective genes -rSS1 (783 bp), rSS2 (468 bp), rSS3 (489 bp), rSS4 (1239 bp), and rSS5 (1131 bp) from M. tuberculosis clinical isolates. lane 3 is negative control. (B) SDS-PAGE analyses of purified recombinant proteins (rSS1, rSS2, SS3, rSS4 and rSS5). Proteins were visualized with Coomassie brilliant blue staining. Abbreviations: WCL: Whole Cell Lysate, FT: Flow Through, P: Purified Protein, M: Marker (Protein).

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