Table 1 Vectors and primers used for cloning of the five novel proteins of Mycobacterium tuberculosis (Mtb) named as rSS1, rSS2, rSS3, rSS4 and rSS5.
S. No. | Gene(s) | Plasmid | Cloning vector (cloning sites) | Primers used for amplification |
|---|---|---|---|---|
1 | rSS1 | pQE30 | pQE-SS1 | F 5′-CGGGATCCATGCCGCTTACACCTGCC-3′ |
(BamHI & HindIII) | R 5′-CCCAAGCTTCTAGTTTTTGCCCCGGTTGAA-3′ | |||
2 | rSS2 | pQE30 | pQE-SS2 | F 5′-CGGGATCCATGACGGCAATCTCGTGCTC-3′ |
(BamHI & HindIII) | R 5′-CCCAAGCTTTTAGCTGGCCGCCAGCTG-3′ | |||
3 | rSS3 | pQE30 | pQE-SS3 | F 5′-CGGGATCCATGAGCGTTGCAAACCTCAAG-3′ |
(BamHI & HindIII) | R 5′-CCCAAGCTTTCACAAAACTCCTCCGGTTGG-3′ | |||
4 | rSS4 | pQE30 | pQE-SS4 | F 5′-CGGGATCCGTG ACG GAC ATG AAC CCG GA-3′ |
(BamHI & HindIII) | R 5′-CCCAAGCTTTCA CGG GCC CCC GGT ACT-3′ | |||
5 | rSS5 | pQE30 | pQE30-SS5 | F 5′-CGGGATCCATGACCAAGAGTCTGCCAGG-3 |
(BamHI & HindIII) | R 5′-CCCAAGCTT# TCAAACCCGGCTAAGGTGC-3 |
