Figure 3: Poly(I:C)-induced IL-1β secretion depends on NLRP3 inflammasome activation in ORS cells. | Scientific Reports

Figure 3: Poly(I:C)-induced IL-1β secretion depends on NLRP3 inflammasome activation in ORS cells.

From: Double-stranded RNA induces inflammation via the NF-κB pathway and inflammasome activation in the outer root sheath cells of hair follicles

Figure 3

(a) ORS cells were pretreated with specific caspase-1 and/or caspase-4 inhibitors for 2 h and then stimulated with poly(I:C) (10 μg/mL) for 24 h. IL-1β secretion in supernatants was assessed using ELISA. (b) ORS cells were transduced with adenoviruses expressing caspase-1- and NLRP3-specific microRNA (miR) and cultured for 3 days. Scrambled microRNA (miR-scr) was used as a control. Knockdown of caspase-1 and NLRP3 was determined by Western blotting. (c,d) After the knockdown of caspase-1 or NLRP3 in ORS cells, cells were further treated with poly(I:C) (10 μg/mL) for 24 h. Supernatants were analyzed for IL-1β by ELISA. Data are presented as means ± SEM. Data were analyzed by one-way ANOVA (n = 3, *p < 0.05, **p < 0.01). Cropped blots were used in this figure and full-length blots are presented in Supplementary Figure 5.

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