Figure 5: Poly(I:C) regulates the translocation and release of HMGB1 in ORS cells. | Scientific Reports

Figure 5: Poly(I:C) regulates the translocation and release of HMGB1 in ORS cells.

From: Double-stranded RNA induces inflammation via the NF-κB pathway and inflammasome activation in the outer root sheath cells of hair follicles

Figure 5

(a) ORS cells were treated with poly(I:C) (10 μg/mL) for the indicated times. Cell extracts were fractionated, and the expression level of HMGB1 was assessed by Western blotting. α-tubulin and laminB were used for determination of the cytoplasmic and nuclear fractions, respectively. The release of HMGB1 in supernatants (SN) was also assessed by Western blotting. (b) ORS cells were treated with poly(I:C) at the indicated concentrations for 24 h. Supernatants were analyzed for HMGB1 by ELISA. Data are presented as means ± SEM (n = 3). Data were analyzed by one-way ANOVA (n = 3, *p < 0.05). Cropped blots were used in this figure and full-length blots are presented in Supplementary Figure 6.

Back to article page