Figure 5: Effects of Sphk1 deletion on endothelial function and intima-media thickness. | Scientific Reports

Figure 5: Effects of Sphk1 deletion on endothelial function and intima-media thickness.

From: Vascular transcriptome profiling identifies Sphingosine kinase 1 as a modulator of angiotensin II-induced vascular dysfunction

Figure 5

(a,b) Tissue organ bath was performed using 2 mm aortic rings (a) or 2nd order mesenteric arteries (b) from WT and Sphk1−/− mice with an assessment of relaxation in response to increasing concentrations of acetylcholine. Vessels were precontracted using concentration of vasoconstricting agent (PGF2α or noradrenaline) required to achieve 90% of maximal contraction as determined by testing increasing doses of certain vasoconstrictor, n = 6/group; (c,d) Masson’s trichrome staining of representative aortas of WT and Sphk1−/− mice (c) and comparison of mean IMT of all groups studied (d), n = 5 for Sham groups, n = 7 for AngII groups; *p < 0.05. WT = wild type; Ach = acetylcholine; SNP = sodium nitroprusside, [M] = molar concentration.

Back to article page