Figure 5: Proteasome inhibitor MG-132 increases both wild-type and mutant TBX1 protein level.
From: A 3 base pair deletion in TBX1 leads to reduced protein expression and transcriptional activity
C2C12 cells and 3T3 cells were transfected with equal concentrations of TBX1-encoding plasmids (the wild-type or the mutant 102delK) and treated with 20uM MG-132 or 10uM E-64 for 8 hours. The TBX1 levels were determined by Western blot (A: C2C12 cells; B: 3T3 cells). GAPDH was used as an internal control. n = 3. Wt: wild-type; Mut: the TBX1102delK mutant; vehicle: DMSO. The full-length image is presented in the Supplementary Figure S4.
