Figure 3: Ibuprofen and production of INSL3 by Leydig cells.
From: Ibuprofen results in alterations of human fetal testis development
(A–C) Ibuprofen and INSL3 production after exposure to ibuprofen during 72 h of human fetal testis culture at (A) 8–12 gestational weeks (GW), (B) 8–9.9 GW, and (C) 10–12 GW. Results are expressed as fold change from the first day of culture (FC to D0). Values are means ± SEM (n = 12–31 testes from 12–31 fetuses). ANOVAs with a random fetus effect were performed using unstructured covariance matrices. A Wilcoxon test was performed for pairwise comparisons. (*p < 0.05). (D) Quantitative RT-PCR for INSL3 was performed on control testes (white bars) and testes treated with 10−5M of ibuprofen (grey bars) for 24 and 48 h. Each column is made up of a pool of 11–15 fetal testes. Each bar represents the mean ± SEM of the fold change in target gene expression relative to RPLP0 reference gene. A non-parametric signed rank Wilcoxon test on paired data was performed (***p < 0.001).
