Figure 6: Ibuprofen and germ cells.
From: Ibuprofen results in alterations of human fetal testis development
(A–C) Representative images of M2A immunostaining in cultured explants of an 8.6-gestational week (GW) human fetal testis. M2A appears brown (3,3′-diaminobenzidine tetrahydrochloride staining), and sections were counterstained with hematoxylin. Scale bar = 100 μm. (D) Numbers of germ cells were determined by counting AMH-negative cells after culturing human fetal testis (n = 7 testes from 7 fetuses) in the presence of 0.01% of DMSO (Control, white bars) or 10−5M (grey bars) or 10−4M (black bars) of ibuprofen. Data are presented as the number of cells per surface area unit (0.01 mm2) (means ± SEM) based on 1 or 2 explants per treatment from 7 fetuses. A non-parametric signed rank Wilcoxon test on paired data was performed. (E) Quantitative RT-PCR for POU5F1, TFAP2C, LIN28A, ALPP and KIT was performed on control testes (white bars) and testes treated with 10−5M of ibuprofen (grey bars) at 24 or 48 h of culture. Each column shows a pool of 11 or 15 fetal testes. Each bar represents the mean ± SEM of the fold change in target gene expression relative to the RPLP0 reference gene. A non-parametric signed rank Wilcoxon test on paired data was performed (*p < 0.05, **p < 0.01; ***p < 0.001).
