Table 2 Primers used for qPCR.
From: Ibuprofen results in alterations of human fetal testis development
Gene | Upstream primer | Downstream primer | Product length (bp) | Annealing temp °C |
|---|---|---|---|---|
ALPP | TCTGGGTACTCAGGGTCTGG | ATCGCTACGCAGCTCATCTC | 101 | 62 |
AMH | CGCCTGGTGGTCCTACAC | GAACCTCAGCGAGGGTGTT | 162 | 67 |
BZRP | GGCTTCACAGAGAAGGCTGT | ACTGACCAGCAGGAGATCCA | 87 | 66 |
COL2A1 | GGCAATAGCAGGTTCACGTACA | CGATAACAGTCTTGCCCCACTT | 57 | 53 |
CYP11A1 | AGACCTGGAAGGACCATGTG | TCCTCGAAGGACATCTTGCT | 435 | 65 |
CYP17A1 | GTGGAGACCACCACCTCTGT | GCTGAAACCCACATTCTGGT | 108 | 67 |
DHH | TGATGACCGAGCGTTGTAAG | GCCAGCAACCCATACTTGTT | 196 | 67 |
HSD3B2 | GCCTGTTGGTGGAAGAGAAG | GCAGGCTCTTTTCAGGAATG | 158 | 62 |
HSD17B3 | TCCTTGGCCTCTCTACTCCA | AGACAGCATATGGGGTCAGC | 125 | 62 |
INSL3 | CCCAGAGATGCGTGAGAAGT | CCAGCCACTGTAGCAACTCA | 229 | 68 |
KIT | TTCTTACCAGGTGGCAAAGG | AAATGCTTTCAGGTGCCATC | 209 | 62 |
LIN28A | AAGCTGCACATGGAAGGGTT | CCGCCTCTCACTCCCAATAC | 138 | 67 |
POU5F1 | TACTCCTCGGTCCCTTTCC | CAAAAACCCTGGCACAAACT | 131 | 67 |
RPLP0 | TCTACAACCCTGAAGTGCTTGAT | CAATCTGCAGACAGACACTGG | 167 | 66 |
RPS20 | AACAAGCCGCAACGTAAAA | ACGATCCCACGTCTTAGAA | 96 | 67 |
SOX9 | AACGCCTTCATGGTGTGG | TCTCGCTCTCGTTCAGAAGTC | 124 | 59 |
SRD5A3 | AGGAATGCCTACATAACAGGGAA | CTCCAAATGGGATCCTGTGGT | 181 | 62 |
STAR | GGCTGGCATGGCCACAGACT | TTGGGCAGCCACCCCTTGA | 162 | 77 |
TFAP2C | CCGGTCCTTGCGGGAGAAGTT | CTGGTTTACTAGGAAATTCGGCTTCACA | 164 | 75 |
