Figure 3: Detection sensitivity.

2 μg of total RNA extracted from Shimen- or HCLV-infected cells were serial 10-fold diluted, and subjected to detection for Shimen RNA using group 1 probes (A) or for HCLV RNA using group 2 probes (B). Absorbance (OD) was measured at 414 nm and normalized to the value of negative control (Ct). Ct: negative control with no template added to the detection system. The inset is the corresponding photograph of the color change. The data represent the mean ± S.D. of three independent experiments.