Figure 4: Detection specificity.

2 μg of total RNA extracted from different viruses infected cells were detected for Shimen RNA using group 1 probes (A), or tested for HCLV RNA using group 2 probes (B). Absorbance (OD) was measured at 414 nm and normalized to the value of negative control (Ct). Ct: negative control with no template added to the detection system. The inset is the corresponding photograph of the color change. The data represent the mean ± S.D. of three independent experiments.