Figure 1: Representative immunoblots, p53 antibody specificity, subcellular enrichment and protein loading controls. | Scientific Reports

Figure 1: Representative immunoblots, p53 antibody specificity, subcellular enrichment and protein loading controls.

From: Sprint-interval but not continuous exercise increases PGC-1α protein content and p53 phosphorylation in nuclear fractions of human skeletal muscle

Figure 1

(a) Representative immunoblots corresponding to total and phosphorylated protein content measured in the nuclear and cytosolic fractions, before (Pre), immediately (+0 h) and 3 h (+3 h) after the CE and SIE trials. p-p38 MAPKThr180/Tyr182: bottom band at ~38 kDa; PHF20: top band at ~105 kDa. No band was detected in the nuclear fractions for p-ACCSer79. (b) Confirmation of the p53 antibody specificity; samples were run beside an untagged full length p53 recombinant human protein and blotted against both total-p53 (positive control) and p-p53Ser15 (negative control) antibodies. S: sample; RP: p53 recombinant protein. (c) Histone H3 and LDHA were used as indicators of cytosolic and nuclear enrichment, respectively. N: nuclear fractions; C: cytosolic fractions. (d) Whole-lane Coomassie blue staining for both nuclear and cytosolic fractions, and (e) histone H3 (nuclear) and GAPDH (cytosolic), were used to verify equal loading between lanes. The immunoblot and whole-lane Coomassie images in this figure were cropped to improve the conciseness and clarity of the presentation.

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