Table 3 Primers used for real-time RT-PCR analyses of mRNA expression.
Gene | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) |
|---|---|---|
AIF | GATTGCAACAGGAGGTACTCCAAGA | GATTTGACTTCCCGTGAAATCTTCTC |
cyt c | GGGCCAAATCTCCATGGTCT | TCTCCCCAGATGATGCCTTT |
DRP1 | CACCCGGAGACCTCTCATTC | CCCCATTCTTCTGCTTCCAC |
MFN2 | CCCCCTTGTCTTTATGCTGATGTT | TTTTGGGAGAGGTGTTGCTTATTTC |
p53 | GTTCCGAGAGCTGAATGAGG | TTATGGCGGGAGGTAGACTG |
PGC-1α | GGCAGAAGGCAATTGAAGAG | TCAAAACGGTCCCTCAGTTC |
p21 | GCAGACCAGCATGACAGATTT | GATGTAGAGCGGGCCTTTGA |
SOD2 | CTGGACAAACCTCAGCCCTA | TGATGGCTTCCAGCAACTC |
cyclophilin | GTCAACCCCACCGTGTTCTTC | TTTCTGCTGTCTTTGGGACCTTG |
GAPDH | AATCCCATCACCATCTTCCA | TGGACTCCACGACGTACTCA |
B2M | TGCTGTCTCCATGTTTGATGTATCT | TCTCTGCTCCCCACCTCTAAGT |
