Figure 3
From: Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleTJE
Decarboxylation and hydroxylation reactions catalyzed by OleTJE and its mutants H85X (A) and I170X (B). Reaction conditions: wild type or mutant enzymes (2 μM), H2O2 (220 μM), and myristic acid (200 μM) in 200 μl desalting buffer were incubated at 30 °C for 16 h. All experiments were performed in duplicate.
