Figure 4: Myristic acid decarboxylation reactions catalyzed by OleTJE when partnered with different redox systems.
From: Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleTJE
Reaction conditions: OleTJE (1 μM), NADPH (5 mM), myristic acid (400 μM), ferredoxin (20 μM), and ferredoxin reductase (20 μM) in 100 μl desalting buffer were incubated at 30 °C for 16 h. All experiments were performed in duplicate.
