Figure 1: The transcriptome analysis of peritoneal macrophages in response to estrogen.

Non-elicited peritoneal macrophages obtained from metaestrous (ME) female mice were analyzed by RNA sequencing after the in vivo treatment with vehicle (time 0, n = 3) or E₂ administration for 3 (n = 3) or 24 h (n = 3). (a) The temporal expression profile of DEGs is represented as heat map (red, high relative expression; black, mean expression; green, low relative expression). (b) By k-means clustering calculations, DEGs were grouped in 4 clusters according to the absolute variation in their expression profile. The number of up (red) and down (green)-regulated genes in each cluster is shown. Gene expression levels are shown in relation to the mean expression value of the DEGs in each cluster (magenta line). (c) Transcription factors (Txf) that bind putative binding sites in up (+) or down (−)-regulated genes are listed for each cluster; the column on the right shows the number of genes with Txf binding sites in their promoters reported as the percentage of the total number of up (+) or down (−)-regulated genes in each cluster. Esr1/2: binding sites for estrogen receptors¹⁶.