Figure 3: Regulation of target gene expression by E2 in isolated macrophages. | Scientific Reports

Figure 3: Regulation of target gene expression by E2 in isolated macrophages.

From: Self-renewal and phenotypic conversion are the main physiological responses of macrophages to the endogenous estrogen surge

Figure 3

Peritoneal macrophages (perMP) and BMDM were grown in culture and assayed for gene expression following E2 administration. (a) Macrophage cells were treated with vehicle (veh, open bars) or increasing concentrations of E2 (10–9, 10−7 and 10−5 M, filled bars) for 3 h. Real time PCR was used to analyze the mRNA levels coding for Vegfα, Angtpl4 and Arg1. (b) Comparison of estrogen action in BMDM cells from WT and ERα-KO (ERKO) mice. The mRNA levels coding for Vegfα, Angtpl4 and Arg1 were analyzed 24 h after the addition of vehicle (veh, open bars) or 10−7 M E2 (filled bars). Data sets for each gene were calculated using the 2−ddCt method with respect to the mean value of the vehicle group. Bars represent mean values ± SEM (n = 4). *p < 0.05; **p < 0.01 versus veh.

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