Figure 4: Validation of E2 target genes in peritoneal macrophages in vivo. | Scientific Reports

Figure 4: Validation of E2 target genes in peritoneal macrophages in vivo.

From: Self-renewal and phenotypic conversion are the main physiological responses of macrophages to the endogenous estrogen surge

Figure 4

Peritoneal macrophages were isolated from female mice at the metaestrous phase (ME) of the estrous cycle or treated with E2 for 3, 24 and 48 h. Females in the estrous phase (E) were also investigated. Real time PCR was performed to detect the mRNAs coding for genes related with cell cycle phases (a) G1/S (CcnD1, E2f1), (b) G2/M (CcnB2, Cdk1, Ube2c; Ki67) and (c) alternative polarization (Arg1, Ym1, CD206, Vegfα). Data sets for each gene were calculated using the 2−ddCt method with respect to the mean value of the control group (ME). Bars represent mean values ± SEM (n = 5–10). *p < 0.05; **p < 0.01; ***p < 0.001 versus ME.

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