Figure 1: Primary and secondary metabolism pathways in F. graminearum and fluorescence tagged proteins. | Scientific Reports

Figure 1: Primary and secondary metabolism pathways in F. graminearum and fluorescence tagged proteins.

From: Structural reorganization of the fungal endoplasmic reticulum upon induction of mycotoxin biosynthesis

Figure 1

The mevalonate pathway enzyme HMG-CoA reductase (Hmr1), and the trichothecene biosynthetic pathway enzymes trichodiene synthase (Tri5), trichodiene oxygenase (Tri4), calonectrin oxygenase (Tri1), as well as Tri14 were tagged at the C-terminus with fluorescent proteins RFP or GFP. Hmr1 catalyzes the synthesis of mevalonate, leading to farnesyl pyrophosphate (FPP), which is utilized by essential metabolic pathways (e.g. biosynthesis of ubiquinone, steroids and terpenes) as well as the trichothecene pathway. After cyclization of FPP by Tri5, oxygenation of the trichodiene product by Tri4 creates the toxic epoxide moiety in isotrichodermol and subsequent pathway intermediates. Further reactions catalyzed by Tri101, Tri11, Tri3, Tri1, and Tri8 generate deoxynivalenol (DON) and 15- acetyldeoxynivalenol (15-ADON). The reason why Tri14 is required for DON synthesis in planta is not known13.

Back to article page