Figure 3: C11orf83 inhibits VSV replication through OAS3-RNaseL system.
From: Mitochondrial C11orf83 is a potent Antiviral Protein Independent of interferon production
(A) qPCR analysis of OAS variants in C11orf83 overexpressed HEK293 cells. **p < 0.01. (B–F) Effects of the loss of OAS3 to rVSV-GFP replication in C11orf83 overexpressed HEK293 cells. HEK293 cells were transfected with pcDNA3.1 (vector) or pcDNA3.1-C11orf83, and 48 hours later cells were transfected with siControl or siOAS3. In the next day, cells were infected with rVSV-GFP (MOI = 1.0) and 12 hours later assays were performed. **p < 0.01. (B,D) Quantitative analysis of GFP positive cells and mean fluorescence intensity in cells by using flow cytometry. (C) Quantitative RT-PCR results shows the relative change of N gene transcription. (E) Quantitative analysis of VSV-induced cells death using Trypan Blue staining. (F) RNA integrity analysis using Agilent Bioanalyzer 2100. (G–J) Effects of the loss of RNase L to rVSV-GFP replication in C11orf83 overexpressed HEK293 cells. HEK293 cells were transfected with pcDNA3.1 (vector) or pcDNA3.1-C11orf83, and 48 hours later cells were transfected with siControl or siRNase L. In the next day, cells were infected with rVSV-GFP (MOI = 1.0) and 12 hours later assays were performed. **p < 0.01. (G,I) Quantitative analysis of GFP positive cells and mean fluorescence intensity in cells by using flow cytometry. (H) Quantitative RT-PCR results shows the relative change of N gene transcription. (J) Quantitative analysis of VSV-induced cells death using Trypan Blue staining.
