Figure 5: C11orf83 inhibits VSV replication through OAS3-RNaseL system in the absence of IFNs production. | Scientific Reports

Figure 5: C11orf83 inhibits VSV replication through OAS3-RNaseL system in the absence of IFNs production.

From: Mitochondrial C11orf83 is a potent Antiviral Protein Independent of interferon production

Figure 5

(A,B) qPCR analysis to the expression of OAS3 in the mRNA level in IFN-deficient Vero (B) and Vero E6 (C) cells. Vero and Vero E6 cells were transfected with pcDNA3.1 (vector) or pcDNA3.1-C11orf83-monkey. **p < 0.01. (C–F) Effects of the loss of OAS3 to rVSV-GFP replication in C11orf83 overexpressed Vero cells. Vero cells were transfected with pcDNA3.1 (vector) or pcDNA3.1-C11orf83-monkey, and 48 hours later cells were transfected with siControl or siOAS3. In the next day, cells were infected with rVSV-GFP (MOI = 0.1) and 12 hours later assays were performed. **p < 0.01. (C,D) Quantitative analysis of GFP positive cells and mean fluorescence intensity in cells by using flow cytometry. (E) Quantitative RT-PCR results shows the relative change of N gene transcription. (F) Quantitative analysis of VSV-induced cells death using Trypan Blue staining.

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