Table 1 Primers for qPCR.
From: Mitochondrial C11orf83 is a potent Antiviral Protein Independent of interferon production
Genes | Primer sequence | |
|---|---|---|
Forward | Reverse | |
C11orf83 | ATGGATTCCTTGCGGAAAAT | ACCATCCAGTTCTTCCTCCA |
N-Gene | CTAAACTACCGGCCAATGAGGAT | TCCGCTAACCTTGCTGAGAT |
OAS1-p42 | ATGATGGATCTCAGAAATACCC | TCAAGCTTCATGGAGAGGG |
OAS1-p44 | ATGATGGATCTCAGAAATACCC | CTAAGCAACCTGGAAACTATAGGATAA |
OAS1-p46 | ATGATGGATCTCAGAAATACCC | TCAGAGGATGGTGCAGGTC |
OAS1-p48 | ATGATGGATCTCAGAAATACCC | TCAGGAGACCTGGGTTCTGTC |
OAS1-p52 | ATGATGGATCTCAGAAATACCC | TTATCTATGATAGGATAGAGGGCATAGA |
OAS2-p69 | ATGGGAAATGGGGAGTCCC | TTAGATGACTTTTACCGGCACTTT |
OAS2-p71 | ATGGGAAATGGGGAGTCCC | CTAGAAGTTTCTTTTAGAATTATTATTCAGGA |
OAS3 p100 | CTTGAGGACTGGATGGATGTTAG | ACTGTTGAGGAGGGTAGAGTAG |
OASL p59 | ATGGCACTGATGCAGGAACTG | CTAACTGGCTGGAAACAGAGCC |
OASL p30 | ATGGCACTGATGCAGGAACTG | TCATAGCAACAGTCCTGTTTCAGG |
RNase L | CCTGGGCCTTCTGAACATT | TGTTCTGGTAGAAATTGCCTCT |
Actin | GGACCTGACTGACTACCTCAT | CGTAGCACAGCTTCTCCTTAAT |
IFN-α | TCCATGAGATGATCCAGCAG | ATTTCTGCTCTGACAACCTCC |
IFN-β | GACGCCGCATTGACCATCTA | CCTTAGGATTTCCACTCTGACT |
IFN-λ1 | CTGGAGGCATCTGTCACCTT | TGGGTTGACGTTCTCAGACA |
IFN-λ2/3 | GCCTCTGTCACCTTCAACCTC | GGAGGGTCAGACACACAGGT |
OAS3(Vero) | CTGGTCTGAGCCTCAAGTTTC | GGCTAACATCCATCCAGTCTTC |
GAPDH(Vero) | GGTGTGAACCATGAGAAGTATGA | GAGTCCTTCCACGATACCAAAG |
GAPDH | ACCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGTA |
