Figure 2: The nuclear Ca2+ transient in neonatal rat ventricular cardiomyocytes (NRVCs) subjected to hypertrophic stimuli.
From: Emerin plays a crucial role in nuclear invagination and in the nuclear calcium transient
(A) Confocal microscopic images of Fluo-4 fluorescence (green) and nuclei (blue). The scale bar is 20 μm. (B) Characterization of cytoplasmic and nuclear Ca2+ transients in NRVCs. Line scan imaging of cytoplasmic (Left) and nuclear (Right) Ca2+ transients in cardiomyocytes. (C) Original recordings of nuclear and cytoplasmic Ca2+ transients of control NRVC. (D,E) Average values of the effects of hypertrophic stimuli on time to peak and half-decay time in the cytoplasmic of NRVCs exposed to vehicle (control; n = 19), Ang II (n = 42), ET-1 (n = 21), or PE (n = 29). (F,G) Average values of the effects of hypertrophic stimuli on time to peak and half-decay time in the nuclei of NRVCs exposed to vehicle (control; n = 19), Ang II (n = 42), ET-1 (n = 21), or PE (n = 29). *P < 0.05 compared to the control. **P < 0.01 compared to the control. †P < 0.001 compared to the control.
