Figure 3: Nuclear structure and the nuclear Ca2+ transient in Emd knockdown neonatal rat ventricular cardiomyocytes (NRVCs).
From: Emerin plays a crucial role in nuclear invagination and in the nuclear calcium transient
(A) Top: Confocal microscopic images of immunostaining for cTnT (red), LAMIN A/C (green) and nuclei (blue) in NRVCs with control scrambled siRNA (siScrambled, left) and siRNA against Emd (siEmd, right). Bottom: Electron microscopic images of NRVCs with control scrambled siRNA (siScrambled, left) and siRNA against Emd (siEmd, right). The scale bar is 5 μm. Red arrow indicates nuclear invagination. (B) Bar graphs showing the percentages of cardiomyocytes with nuclear invagination in NRVCs exposed to siScrambled (n = 69) and siEmd (n = 62) for 48 hours in culture. The data are mean ± SE. (C) Bar graphs showing the percentages of cardiomyocytes with nuclear size in NRVCs exposed to siScrambled (n = 69) and siEmd (n = 62) for 48 hours in culture. The data are mean ± SD. (D) Bar graph showing nuclear volumes in NRVCs exposed to siScrambled (n = 95) or siEmd (n = 138). The data are mean ± SD. (E) Original recordings of the nuclear Ca2+ transient of NRVCs with siScrambled (n = 25) and siEmd (n = 29). (F,G,H) Average values of the effects of the Emd knockdown on F/F0, time to peak, and half-decay time in the nuclei of NRVCs. *P < 0.05 compared to the control. **P < 0.01 compared to the control. †P < 0.001 compared to the control.
