Figure 2 | Scientific Reports

Figure 2

From: Expression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase

Figure 2

(A) Schematic illustration of the expression vector (Amp R: ampicillin-resistance gene, large, medium, and small chain of the CODH gene sequence encoding cox L, cox M, and cox S, Lac I: Lac I repressor gene sequence), (B) SDS-PAGE (M: pre-stained protein standard, L: cell lysate, FT: flow through fraction, W: wash fraction with 20 mM imidazole, E: eluted protein with 250 mM imidazole) of the purified recombinant PsCODH using a 12 % acrylamide gel under denaturing conditions, and (C) determination of the molecular mass of the gross purified recombinant PsCODH using size exclusion chromatography. Thyroglobulin (699 kDa), ferritin (440 kDa), aldolase (158 kDa), and conalbumin (75 kDa) were used as reference proteins.

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