Figure 3: UV-vis absorption spectra of the recombinant PsCODH.

Spectra are of the native PsCODH (
), the CO-reduced PsCODH (2 min CO purging [
]), and the sodium dithionite (DT)-reduced PsCODH (1 min after mixing [
]). The spectra were recorded in elution buffer containing 250 mM imidazole (pH 8.0). The base-line was adjusted using an elution buffer before recording.