Figure 6: Relative activity of the recombinant PsCODH using BFG, a kind of CO-containing waste gas from the steelmaking process.

The activity was assayed at pH 6.5 (200 mM phosphate buffer) and 30 °C using thionine acetate as the electron acceptor (20 μM). The relative activity was calculated based on the percentage of activity when using pure CO as the substrate. The experiments were performed in triplicate