Table 2 Kinetic parameters and specific activities of aerobic CODHs.

From: Expression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase

 

kcat (s−1)

Km (mM)

Specific activity (U mg−1)*

Electron acceptor

Assay condition

References

Bradyrhizobium japonicum

  

0.058

PMS-NBT

pH 7.5

24

Oligotropha carboxidovorans

93.3

0.011

 

MB

pH 7.2, 30 °C

19

Pantoea species YR343

12.97

0.065

0.86

TA

pH 6.5, 30 °C

This study

  1. *1 U of enzyme oxidized 1 μmol of CO to CO2 per minute.
  2. The experiments were performed in triplicate.
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