Figure 1: ANGPTL4 attenuates colonic inflammation. | Scientific Reports

Figure 1: ANGPTL4 attenuates colonic inflammation.

From: Angiopoietin-like 4 Mediates Colonic Inflammation by Regulating Chemokine Transcript Stability via Tristetraprolin

Figure 1

(a–e) Images of colon samples (a) Representative colon sections stained with hematoxylin and eosin (H&E) (b). (c) Disease activity index (DAI) of mice assessed every alternate day over the treatment regimes. Scoring criteria can be found in Supplementary Table S1. (d) Percentage of Ly6G+ and CD68+ cells per field of view. Total DAPI-stained nuclei were taken as the total cell number. Microscopic views from 5 different sections were numerated. (e) Representative FACS histograms for Ly6G expression (neutrophils) from ANGPTL4+/+ and ANGPTL4−/− littermates for the indicated treatments. (f) Representative FACS analysis of the colons from ANGPTL4+/+ and ANGPTL4−/− mice double stained for F4/80 (macrophages) and CD11b (macrophages, monocytes, granulocytes, NK cells, dendritic cells) for each of the treatments. (g) Relative mRNA levels of pro-inflammatory cytokines (TNF-α, IL-6 and IFN-γ) from ANGPTL4+/+ and ANGPTL4−/− mice at the endpoint. For the Veh, DSS and SA groups, n = 20 mice were used for each treatment. The Mann–Whitney U test was used. *p < 0.05; **p < 0.01.

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