Figure 3: Intrinsic role of colonic ANGPTL4 in leukocytes infiltration during DSS.

(a,b) Graphical illustrations depicting the experimental setup for bone marrow transplantation (BMT). CD4+ and CD8+ depleted bone marrow cells from age- and gender-matched ANGPTL4^+/+ or ANGPTL4^−/− donors were transplanted into γ-irradiated ANGPTL4^+/+ wild-type recipient (WT IR). Chimera BMT ANGPTL4^+/+ and BMT ANGPTL4^−/− denote WT IR transplanted with bone marrow cells from ANGPTL4^+/+ and ANGPTL4^−/− donor, respectively. Graph showed the number of CD45⁺ cells from spleen of indicated mice (Mean ± S.D.; n = 6 mice per group). (c,d) Representative images of the femur (c) and spleen (d) from indicated mice (WT and WT IR) and chimeras (BMT ANGPTL4^+/+ and BMT ANGPTL4^−/−). Inserts in (c) showed the bone marrow cell pellet before and after red blood cell lysis. Graph in (c; left panel) showed the number of bone marrow cells from femur of indicated mice (Mean ± S.D.; n = 6 mice per group). (e) Representative agarose gel image of genotype PCR products from CD45⁺ splenic cells from indicated mice. (f) Graphs showing the disease activity index (DAI; left panel), macroscopic score (middle panel) and histological score (right panel) of indicated mice treated with vehicle or 2% DSS. Individual scoring criteria can be found in Supplementary Tables S1–S3. (g) Representative hematoxylin and eosin (H&E) images of colon section from vehicle and DSS treated mice post BMT. (h) Representative FACS analysis for CD11b⁺ and F4/80⁺ cells from colons of BMT ANGPTL4^+/+ and BMT ANGPTL4^−/− chimeras treated with vehicle or DSS. n = 6 per group. The Mann-Whitney U test was used. *p < 0.05; **p < 0.01 and ***p < 0.001. n.s. denotes not significant.