Figure 5: ANGPTL4 deficiency increases the infiltration of immune cells. | Scientific Reports

Figure 5: ANGPTL4 deficiency increases the infiltration of immune cells.

From: Angiopoietin-like 4 Mediates Colonic Inflammation by Regulating Chemokine Transcript Stability via Tristetraprolin

Figure 5

(a) Relative mRNA levels of indicated chemokines in colon tissues from ANGPTL4+/+ and ANGPTL4−/− mice treated with Veh, DSS or SA. (b) Relative expression (left panel) and immunoblot analysis (right panel) of ANGPTL4 and TTP levels in iCECs when stimulated with DSS, SA, IL-1β, TNF-α, HC or NaBu. (c) Heatmap displaying log-transformed relative expression levels of indicated chemokine mRNA transcripts in iCECCtrl, iCECTTP and iCECANGPTL4 when stimulated with DSS, SA, IL-1β, TNF-α, HC or NaBu. (d) Decay curves of IL-1β, IL-10, IL-17, CCL2, CCL11 and CXCL10 in iCECANGPTL4, iCECTTP or iCECCtrl, following actinomycin D treatment after NaBu stimulation. (e) Transwell migration assay measuring the percentage change in relative fluorescence intensity of THP1 migration in response to chemokines secreted by iCECCtrl, iCECTTP and iCECANGPTL4 (bottom panel). Five independent experiments (n = 5) were performed in triplicate. The Mann–Whitney U test was used. *p < 0.05, **p < 0.01, ***p < 0.001.

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