Figure 4: TPPPS reduces viral shedding and viral loads in congenitally ALV-J-infected chickens. | Scientific Reports

Figure 4: TPPPS reduces viral shedding and viral loads in congenitally ALV-J-infected chickens.

From: Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity

Figure 4

Chickens in groups ALV–TPPPS (red) and ALV (blue) were orally administered for 2 weeks with TPPPS and PBS, respectively. Non-infected chickens treated with PBS served as group Mock (black). The cloacal swabs (A) of six chickens and the bursa (B), thymus (C), and spleen (D) tissues of three chickens were collected at 7, 14, 21, 28, 35, 42, 49, and 56 dph, and the viral titers were determined by ELISA. S/P value was calculated by the following equation: S/P = (Sample mean − Negative control mean)/(Positive control mean − Negative control mean). The transverse line in the histogram represents the cut-off point. The tissue sections of thymuses, bursas, and spleens from 14-day-old chickens in each group were prepared for viral localization through the immunofluorescence histochemical staining using the ALV-J monoclonal antibody JE9 (200×; E). The images shown represent three animals from three independent experiments. Scale bar, 50 μm.

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