Table 1 Data collection and refinement statistics for AtClpC1 NTD and AtClpD NTD.
Parameter | AtClpC1 NTD | AtClpD NTD |
|---|---|---|
Data Collection | ||
Beamline | RRCAT-BL21 | ESRF-BM14 |
Detector type | Marmosaic 225 mm CCD | Marmosaic 225 mm CCD |
Wavelength (Å) | 0.9794 | 0.9537 |
Data collection temperature (K) | 103 | 100 |
Space group | P212121 | P21 |
a, b, c (Å) | 40.10, 44.29, 97.56 | 38.08, 37.31, 99.98 |
α, β, γ (°) | 90, 90, 90 | 90, 96.08, 90 |
Resolution (Å) | 17.54–1.20 (1.26–1.20) | 37.87–1.60 (1.63–1.60) |
Rmeas (%) | 7.5 (63.4) | 8.8 (78.1) |
I/σI | 9.4 (2.7) | 9.8 (2.2) |
CC(1/2) (%) | 99.8 (80.5) | 99.5 (71.7) |
Total number of reflections | 262145 | 351287 |
Mosaicity (°) | 0.43 | 0.41 |
Completeness (%) | 98.4 (100) | 100 (100) |
Multiplicity | 4.8 (4.7) | 4.2 (4.1) |
Wilson B-factor (Å2) | 11.9 | 18.2 |
Matthews coefficient (Å3/Da) | 2.66 | 2.00 |
Solvent content (%) | 53.75 | 38.63 |
No. of molecules in ASU | 1 | 2 |
Refinement | ||
No. of unique reflections | 54126 | 37033 |
Rwork/Rfree (%) | 15.6/17.5 | 20.5/22.3 |
Total no. of non-H atoms | 1459 | 2450 |
No. of water molecules | 130 | 104 |
No. of ligands | 5 phosphate ions | 0 |
Mean B-factor (Å2) | 18.0 | 23.0 |
R.m.s. deviations | ||
Bond lengths (Å) | 0.016 | 0.012 |
Bond angles (°) | 1.931 | 1.370 |
Ramachandran plot values (%) | ||
Favoured/Outliers | 100/0 | 100/0 |
