Figure 3: Retention of MSCs and TSCs after transplantation.

(A) The retention of injected cells was detected by the percentage of GFP⁺ in all the cells in every field under fluorescent microscopy, and nuclei were counterstained with DAPI. (B) Quantification of GFP⁺ cells to total cells showed that the retention of TSCs was higher than the MSCs (*P < 0.05) (Table S1). (C) The co-expression of GFP and Tunel indicated the apoptosis of implanted cells in vivo. (D) Quantification of co-expression of Tunel and GFP cells showed no difference between TSCs and MSCs in apoptosis after transplantation (Table S3). (E) The co-expression of GFP and pH3 indicated the proliferation of implanted cells in vivo. (F) Quantification of co-expression of pH3 and GFP cells showed that TSCs exhibited more proliferation than MSCs in vivo (*P < 0.05) (Table S2). n = 6 for each group. Data are depicted as mean ± SD.