Figure 5: Phylogenetic tree of galE1 and galE2 alleles from N. meningitidis.

The phylogenetic tree was constructed using the Neighbor-Joining method with 1000 bootstraps and evolutionary distances were calculated by the maximum composite likelihood method. In order to generate the tree, the alignment of the nucleotides was performed using MUSCLE after trimming the galE1 and galE2 alleles to the last 615 nucleotides. The colours indicate the functionality of the protein associated with the alleles; the filled and empty circles indicates galE1 and galE2 alleles respectively. The asterisks indicate galE1 alleles (given in brackets) from cnl isolates.