Table 1 Analysis of UDP-Glucose and UDP-GlcNAc 4-epimerase activity of GalE alleles and their mutants.
Enzyme | Substrate | Equilibrium ratio UDP-Glc:UDP-Gal | Equilibrium ratio UDP-GlcNAc:UDP-GalNAc |
|---|---|---|---|
Gonococcal GalE_17 | UDP-Glucose | 39:61 | — |
UDP-GlcNAc | — | 32:68 | |
GalE_17 (S299F) | UDP-Glucose | 59:41 | — |
UDP-GlcNAc | — | 99.2:0.8 | |
Meningococcal GalE_2 | UDP-Glucose | 63:37 | — |
UDP-GlcNAc | — | 99.5:0.5 | |
GalE_2 (F300S) | UDP-Glucose | 67:33 | — |
UDP-GlcNAc | — | 73:27 | |
GalE_236 | UDP-Glucose | 23:77 | — |
UDP-GlcNAc | — | 25:75 |
