Figure 7: Complement inhibition assays. | Scientific Reports

Figure 7: Complement inhibition assays.

From: Immune complexes in chronic Chagas disease patients are formed by exovesicles from Trypanosoma cruzi carrying the conserved MASP N-terminal region

Figure 7

51Cr labelled E, T or A forms were treated with 10% heat inactivated sera from chagasic patients diagnosed as digestive, cardiopathic or asymptomatic. After incubation with 1/18 dilution human complement (CH50) and 1 μg/100 μl of MASP SP peptide (a) and EVs from E, T and A forms (b) at 37 °C for 1 h. Non-inactivated pooled sera from chagasic patients was used as a positive control and a pool of inactivate sera without supplemented complement as a negative control.

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