Figure 4: Detection of HA-DOR in brain sections with immunofluorescent staining. | Scientific Reports

Figure 4: Detection of HA-DOR in brain sections with immunofluorescent staining.

From: One-step generation of mice carrying a conditional allele together with an HA-tag insertion for the delta opioid receptor

Figure 4

Brain sections from DORwt/wt, HA-DORfloxETPP/floxETPP, HA-DORflox/flox and HA-DORflox/flox:Nestin-Cre mice were stained with anti-HA antibody and fluorescence-conjugated secondary antibody (red). Cell nuclei were labeled with Hoechst 33342 (blue). Representative confocal images were shown. (A) olfactory bulb, (B) layer V (internal pyramidal layer) of the cortex, (C) pyramidal cell layer of the hippocampal region CA1, (D) basolateral amydala, (E) caudate putamen and (F) spinal cord. Scale bars represent 20 μm.

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