Figure 3: Epithelial LTβR is not required for mTEC proliferation and apoptosis. | Scientific Reports

Figure 3: Epithelial LTβR is not required for mTEC proliferation and apoptosis.

From: Epithelial LTβR signaling controls the population size of the progenitors of medullary thymic epithelial cells in neonatal mice

Figure 3

(a,b) After the surface staining, mTECs (CD45−EpCAM+UEA-1+Ly51−) from each group were intracellularly stained with Ki-67 for comparing their proliferation ability. Representative plots from mice at various time points are shown (a). The percentages of Ki-67 positive cells in mTECs of each group are plotted (b). (c and d) Intracellular staining of active caspase 3 in mTECs was used to distinguish the apoptosis cells in Ltbrfl/flK14Cre and Ltbrfl/+K14Cre control mice. Representative plots from mice at various time points are shown (c). The percentages of active caspase 3 positive cells in mTECs of each group are plotted (d). All experiments have been repeated for more than 3 times with at least 3 mice per group each time. An unpaired two-tailed Student’s t-test is used. No significant difference was found between comparing groups.

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