Figure 5: Deficiency of epithelial LTβR results in impaired negative selection. | Scientific Reports

Figure 5: Deficiency of epithelial LTβR results in impaired negative selection.

From: Epithelial LTβR signaling controls the population size of the progenitors of medullary thymic epithelial cells in neonatal mice

Figure 5

(a) OT-I BM cells were depleted of mature T cells and transferred into lethally irradiated recipients mice as indicated. 6 wk later, OT-I thymocyte development was analyzed by flow cytometry. Total thymocytes were first analyzed with CD4 and CD8 (up row) and then OT-I thymocyte gated with Vα2 and Vβ5 from CD8 single positive T cells (middle row). After gating OT-I thymocyte (CD4−CD8+Vα2+Vβ5+), T cell maturation was determined by CD24 (bottom row). Representative profiles from mice of indicated genotypes are shown. (b) Statistical analysis shows the pooled data from three independent experiments with 6–7 mice each group. (c,d) The proportion of splenic OT-I (CD4−CD8+Vα2+Vβ5+) was plotted (c) and the statistical analysis shows the pooled data from three independent experiments with 6–7 mice each group (d). The statistic results are shown as mean ± SD. An unpaired two-tailed Student’s t-test is used: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. RIP, rat insulin promoter.

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