Figure 3: Association of nELAVs with the APP pre-mRNA. | Scientific Reports

Figure 3: Association of nELAVs with the APP pre-mRNA.

From: Neuronal ELAVL proteins utilize AUF-1 as a co-partner to induce neuron-specific alternative splicing of APP

Figure 3

(A) Localization of primers used for the detection of APP/App pre-mRNAs (arrows; APPI/AppI: Forward I and Reverse I; APPII/AppII: Forward II and Reverse II). Boxes indicate exons and bold lines introns. (B–D) Nuclear extracts prepared from human SK-N-SH (B), human SH-SY5Y (C) and mouse Neuro-2a (D) cells were immunoprecipitated with a mouse anti-ELAVL antibody or mouse IgGs as a control. RNA was isolated from immunoprecipitates, as well as their supernatants and analyzed by semi-quantitative and quantitative RT-PCR, respectively, using specific primers against human or mouse APP pre-mRNA (arrows) and intronic GAPDH. Minus RT lanes are included as controls. Note that APP pre-mRNA was detectable in the immunoprecipitate only when lysates from cells expressing nELAVLs were used. Bars in graphs depict mean ± standard deviation of three independent experiments (*P < 0.5, **P < 0.01).

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