Figure 1: Generation of a bioengineered tooth using embryonic canine tooth germ-derived cells under subrenal capsule transplantation.

(A) Schematic representation of the generation of reconstituted tooth germ. (Illustration by R.N.) (B) Photograph (left) and histological image obtained by HE staining (centre) of permanent first molar tooth germ (M1) and phase contrast image of reconstituted tooth germ on organ culture day 2 (right). Epi, epithelial tissue; Mes, mesenchymal tissue. (C) Micro-CT images of natural tooth germ at 8 and 12 weeks after subrenal capsule transplantation. Three-dimensional (3D) images are shown in the upper column, and cross-sectional views are shown in the lower column (left). Histological analysis of the bioengineered tooth 12 weeks after subrenal capsule transplantation (right). Boxes indicate the area shown at higher magnification in the lower panels. (E), enamel; (D), dentin. (D) Bioengineered tooth germ was reconstituted using single cells derived from molar tooth germ. Micro-CT images (left) and histological image (right) of the bioengineered tooth 8 weeks after subrenal capsule transplantation. 3D images are shown in the upper column, and cross-sectional views are show in the lower column. Boxes indicate the area shown at higher magnification in the lower panels. (E), enamel; (D), dentin.