Figure 3: SEM and EDX analysis in the bioengineered tooth.

SEM image of enamel (A) and dentin (B) of the natural tooth, the erupted tooth formed by the transplantation of natural tooth germ and the bioengineered tooth. Boxes indicate the area shown at higher magnification in the centre panels. To analyse the structure of the enamel rod and dentin tube, the tooth was treated with 40% phosphoric acid for 10 sec and sodium hypochlorite for 15 sec. The surface composition of each tooth was analysed by EDX. C, carbon; O, oxygen; P, phosphorus; Ca, calcium.