Figure 4: Experimental tooth movement in the canine model.

(A) Schematic representation of orthodontic movement of the natural tooth, the erupted tooth formed by transplantation of natural tooth germ and the bioengineered tooth. (Illustration by R.N.) (B) Oral photograph of the orthodontic appliance designed for the canine jawbone. The erupted teeth were continuously loaded with 10 gf of horizontal orthodontic force (from the buccal side to the lingual side) for 30 days using an orthodontic appliance. Arrowhead, erupted tooth; arrow, direction of orthodontic force. (C) CT images of the tooth movement of the erupted tooth formed by transplantation of natural tooth germ and the bioengineered tooth before orthodontic treatment (left panels, blue) and after orthodontic treatment (centre panels, red). Merged images before and after orthodontic treatment are shown (right panels).